Protocols
SDS-PAGE
Western Blot
Faellung von Proteinen
IF Prep cells tissues
RNA isol large scale
Agarose gel extraction
RNA-Isolierung
Site-directed Mutagenesis
Competent bacteria and transformation
Long Range PCR TripleMaster System
Partial trypsin digestion of cornified envelopes
Myko-PCR
Immunfluoreszenz
Isolation murine keratinocytes
Plasmid preparation
Culture of HM1-cells with feeders
Culture of HM1-cells
Chromosome FISH
Superovulation matings BCI
Southern Blotting
Ca-phosph transfection
Nucleofection MDCK II
Molecular cloning
Cryo In situ hybridization
Immunprecipitation
Tissue fixation and embedding
Whole mount in situ hybridization
Transfektion von Säugerzellen
Culture of HM1-cells with FCS
Mirus keratinocyte transfection
Herstellung von Mowiol
Antigen Retrieval
Kultivierung von Saeugerzellen
Behandlung von Zellen mit Ciprofloxacin
M2 Medium
Mouse House Protocols
PCR
Dialyse
cDNA-Synthese SuperScript
Isolierung genomischer DNA aus Zellen und Geweben high quality
Isolierung genomischer DNA fuer PCR
Proteinbestimmung
Northern Blotting
RNA Isolation
LIF production
Purification of phage DNA from liquid cultures
Two-dimensional gel electrophoresis
Creating dA overhangs with Taq
REZEPT für Ivomectin
Silan coating of slides
Hybrid selection assay
Whole mount immunocytochemistry
ZnSo4 stain of SDS PAGE gels
Proteinexpression
Total protein extracts prep
Ammonsulfatfaellung
HiPerFect Transfection Reagent Handbook,
siRNA Transfection
Epid Sheet Prep
Cornified envelope prep
Bradford
ProLongGold Handhabung Invitrogen
Vorbereitung von Deckglaeschen
Primer loesen
DNA Faellung fuer Transfektionsexperimente
yellow blue tips
Aliquoting ultra-pure water
Buffers pH Meter
dNTPs
Cell proliferation assay Cell Signaling
Culture mouse keratinocytes
Fixation FISH
GFP-Trap,
Synchronisation HeLa HEK293
Transfection Keratinocytes Lipofectamin
mouse primary keratinocytes
Poly L Lysin Beschichtung
GFP-Pulldown Protokoll
E coli DH5alpha NEB
3T3-J2 Feeder culture
Zelllysate Keratinocyten
RNA qPCR Standardprotokoll
siRNA reverse trsf keratinoc
XFect protocol
Immunhistochemie Faerbung
Whole mount Immunohistochem embroys
Hoefer 2D Quick Start Guide
Sample preparation for Colony PCR